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Proceedings Paper

Bi-photon imaging and diagnostics using ultra-small diagnostic probes engineered from semiconductor nanocrystals and single-domain antibodies
Author(s): Hilal Hafian; Alyona Sukhanova; Patrick Chames; Daniel Baty; Michel Pluot; Jacques H. M. Cohen; Igor R. Nabiev; Jean-Marc Millot
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Paper Abstract

Semiconductor fluorescent quantum dots (QDs) have just demonstrated their numerous advantages over organic dyes in bioimaging and diagnostics. One of characteristics of QDs is a very large cross section of their twophoton absorption. A common approach to biodetection by means of QDs is to use monoclonal antibodies (mAbs) for targeting. Recently, we have engineered ultrasmall diagnostic nanoprobes (sdAb–QD) based on highly oriented conjugates of QDs with the single-domain antibodies (sdAbs) against cancer biomarkers. With a molecular weight of only 13 kDa (12-fold smaller than full-size mAbs) and extreme stability and capacity to refolding, sdAbs are the smallest functional Ab fragments capable of binding antigens with affinities comparable to those of conventional Abs. Ultrasmall diagnostic sdAb–QD nanoprobes were engineered through oriented conjugation of QDs with sdAbs. This study is the first to demonstrate the possibility of immunohistochemical imaging of colon carcinoma biomarkers with sdAb–QD conjugates by means of two-photon excitation. The optimal excitation conditions for imaging of the markers in clinical samples with sdAb–QD nanoprobes have been determined. The absence of sample autofluorescence significantly improves the sensitivity of biomarker detection with the use of the two-photon excitation diagnostic setup.

Paper Details

Date Published: 24 October 2012
PDF: 8 pages
Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85480R (24 October 2012); doi: 10.1117/12.960493
Show Author Affiliations
Hilal Hafian, Univ. de Reims Champagne-Ardenne (France)
Alyona Sukhanova, Trinity College Dublin (Ireland)
Moscow Engineering Physics Institute (Russian Federation)
Patrick Chames, INSERM (France)
Institut Paoli-Calmettes (France)
Zix Marsellie Univ. (France)
Daniel Baty, INSERM (France)
Institut Paoli-Calmettes (France)
Aix Marsellie Univ. and CNRS (France)
Michel Pluot, Univ. de Reims Champagne-Ardenne (France)
Jacques H. M. Cohen, Univ. de Reims Champagne-Ardenne (France)
Igor R. Nabiev, Trinity College Dublin (Ireland)
Moscow Engineering Physics Institute (Russian Federation)
Jean-Marc Millot, Univ. de Reims Champagne-Ardenne (France)

Published in SPIE Proceedings Vol. 8548:
Nanosystems in Engineering and Medicine
Sang H. Choi; Jin-Ho Choy; Uhn Lee; Vijay K. Varadan, Editor(s)

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