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Proceedings Paper

Three-Dimensional Analysis of Biological Specimens Utilizing Image Processing Techniques
Author(s): David A. Agard; John W. Sedat
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Paper Abstract

Examination of biological structures at the level of whole cells presents several major problems that can be solved using two- and three-dimensional reconstruction and image enhancement techniques. Presented here are first steps towards realizing this goal of cellular tomography. General methodologies for extraction of additional information from biological objects using median and bandpass filtration and constrained deconvolution procedures are described. These image processing techniques are ideally suited for studying large non-crystalline objects by either optical or electron microscopy. The ultrastructural architecture of Drosophila sperm heads has been investigated, revealing that these examples of highly-condensed DNA have a defined arrangement based on helical principles.

Paper Details

Date Published: 21 November 1980
PDF: 8 pages
Proc. SPIE 0264, Applications of Digital Image Processing to Astronomy, (21 November 1980); doi: 10.1117/12.959792
Show Author Affiliations
David A. Agard, University of California (United States)
John W. Sedat, University of California (United States)

Published in SPIE Proceedings Vol. 0264:
Applications of Digital Image Processing to Astronomy
Denis A. Elliot, Editor(s)

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