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Proceedings Paper

Laser Microfluorometer For Fluorescence Decay Measurements In Biology
Author(s): A. Andreoni; A. Longoni; G. Bottiroli; G. Prenna
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Paper Abstract

A laser microfluorometer is described which is particularly suitable for the measurement of fluorescence decays in microscopic biological samples. The apparatus makes use of (1) a nitrogen pumped dye laser of special design as the excitation source, (2) a microscope system particularly suitable for fluorescence microscopy and (3) an electronic signal averager. The main properties of this instrument include high temporal resolution (~0.2 ns) and high spatial resolution (~0.5μm). This instrument is now being used to study the fluorescence properties of complexes formed by acridine dyes with deoxyribonucleic acid (DNA). Preliminary results indicate that specific information about DNA base-pair sequences and DNA microscopic structure in chromosomes can be obtained.

Paper Details

Date Published: 10 April 1977
PDF: 13 pages
Proc. SPIE 0099, 3rd European Electro-Optics Conf, (10 April 1977); doi: 10.1117/12.955338
Show Author Affiliations
A. Andreoni, Istituto di Fisica del Politecnico (Italy)
A. Longoni, Istituto di Fisica del Politecnico (Italy)
G. Bottiroli, Universita di Pavia (Italy)
G. Prenna, Universita di Pavia (Italy)


Published in SPIE Proceedings Vol. 0099:
3rd European Electro-Optics Conf
Herbert A. Elion, Editor(s)

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