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Proceedings Paper

Development Of A Solid State Microscope
Author(s): B. Jaggi; B. Palcic
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Paper Abstract

A Solid State Microscope (SSM) has been designed and is being developed in order to improve and optimize spatial, photometric and spectral resolution for quantitative microscopy. The SSM is an opto-electronic device for scanning and viewing microscopic objects in the visible light spectrum. Using Kohler illumination, pulsed light is transmitted through the microscope sample and focused by a single objective lens. The objective magnifies and projects the image onto a large area charge-coupled device (CCD) located at the intermediate focal plane (PIP). Sample scanning procedures and optical considerations require a CCD array of greater than 1000 x 1000 picture elements, with each element having a sensing area of approximately 7 μm x 7 μm. The signals from the picture elements are directly digitized and mapped on a one-to-one basis into a large frame memory at a rate of 20 Mpixels/s. The full digital image is continuously displayed in real-time at a rate of 60 frames/s on a gray scale monitor. The images stored in frame memory are accessed by workstation for quantitative measurements. The rationale for these specifications and in particular, how CCD characteristics relate to the optics and image display are discussed. Of particular importance is the sampling density which has been experimentally determined. The data shows that oversampling the image 3 to 4 times will be optimal for this design and a spatial resolution of at least 0.4 μm can be expected at a field of view of 1.4 Mpixels.

Paper Details

Date Published: 1 May 1989
PDF: 11 pages
Proc. SPIE 1090, Medical Imaging III: Image Formation, (1 May 1989); doi: 10.1117/12.953190
Show Author Affiliations
B. Jaggi, B.C. Cancer Research Centre (Canada)
B. Palcic, B.C. Cancer Research Centre (Canada)


Published in SPIE Proceedings Vol. 1090:
Medical Imaging III: Image Formation
Samuel J. Dwyer; R. Gilbert Jost; Roger H. Schneider, Editor(s)

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