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Proceedings Paper

Fiber Optic Immunochemical Sensors For Continuous Monitoring Of Hapten Concentrations
Author(s): W. Greg Miller; F. Philip Anderson
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Paper Abstract

We describe a fiber optic sensor based on a homogeneous fluorescence energy transfer immunoassay which operates in a continuous, reversible manner to quantitate the anticonvulsant drug phenytoin. B-phycoerythrin-phenytoin and Texas Red labeled anti-phenytoin antibody were sealed inside a short length of cellulose dialysis tubing which was cemented to the distal end of an optical fiber. When the sensor was placed into a solution of phenytoin, the drug crossed the dialysis membrane, displaced a fraction of the B-phycoerythrin-phenytoin from the antibody, and produced a change in fluorescence signal which was measured with a fiber optic fluorometer. The sensor had a concentration response of 5 to 500μmo1/L phenytoin with a response time of 5 to 15 min and precision of <2.5% CV. The chemical kinetics of the antibody-hapten indicator reaction were modeled mathematically and simulation showed that response time in the minutes range can be achieved when the dissociation rate constant is greater than approximately 10-3 sec-1. The dissociation rate constant influences the time to reach equilibrium and the unbound P* concentration range available for instrumental measurement. The ratio of the labeled and unlabeled hapten dissociation rate constants influences the analyte concentration range to which the sensor will respond.

Paper Details

Date Published: 15 June 1989
PDF: 7 pages
Proc. SPIE 1067, Optical Fibers in Medicine IV, (15 June 1989); doi: 10.1117/12.952098
Show Author Affiliations
W. Greg Miller, Virginia Commonwealth University (United States)
F. Philip Anderson, Virginia Commonwealth University (United States)

Published in SPIE Proceedings Vol. 1067:
Optical Fibers in Medicine IV
Abraham Katzir, Editor(s)

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