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Proceedings Paper

Flow Cytometric Ultrasensitive Molecular Detection And Applications
Author(s): James H. Jett; Dinh C. Nguyen; John C. Martin; Richard A. Keller
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Paper Abstract

The traditional domain of flow cytometry has been the measurement of properties of discrete biological particles - primarily cells or chromosomes. In actuality, most flow cytometric fluorescence measurements of cellular properties are determinations of the amount of a specific type of molecule such as DNA or a cell surface antigen. Since the early developments in flow cytometry, there has been a continuing quest for more sensitive instruments and for techniques to detect and quantitate lower levels of cellular components. The effort described here is directed at increasing the sensitivity of molecular detection by laser induced fluorescence to the point that single molecules can be both detected and identified.

Paper Details

Date Published: 13 June 1989
PDF: 4 pages
Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); doi: 10.1117/12.951912
Show Author Affiliations
James H. Jett, Los Alamos National Laboratory (United States)
Dinh C. Nguyen, Los Alamos National Laboratory (United States)
John C. Martin, Los Alamos National Laboratory (United States)
Richard A. Keller, Los Alamos National Laboratory (United States)

Published in SPIE Proceedings Vol. 1063:
New Technologies in Cytometry
Gary C. Salzman, Editor(s)

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