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Proceedings Paper

Analysis Of PCNA (Proliferating Cell Nuclear Antigen) In The Cell Cycle And Application To Flow Cytometry
Author(s): Goran Roos; Goran Landberg; Kevin F. Sullivan; Eng M. Tan
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Paper Abstract

PCNA also called cyclin, is a nuclear protein and its synthesis is directly correlated to cell proliferation. At least two intracellular populations of PCNA/cyclin exist, one of which is tightly associated with DNA replication sites. This S-phase associated protein can be used as an antigenic marker for cell growth and more specifically for the S-phase. Data from clinical studies indicate that the expression of PCNA/cyclin , evaluated by immunocytochemistry or flow cytometry, can be a useful diagnostic marker for proliferating cells. In the future, the characterization of tumors might include multiparameter analysis of the simultaneous expression of multiple cell proliferation markers at different cell cycle stages.

Paper Details

Date Published: 13 June 1989
PDF: 6 pages
Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); doi: 10.1117/12.951900
Show Author Affiliations
Goran Roos, W.M. Keck Autoimmune Disease Center (United States)
Goran Landberg, W.M. Keck Autoimmune Disease Center (United States)
Kevin F. Sullivan, W.M. Keck Autoimmune Disease Center (United States)
Eng M. Tan, W.M. Keck Autoimmune Disease Center (United States)

Published in SPIE Proceedings Vol. 1063:
New Technologies in Cytometry
Gary C. Salzman, Editor(s)

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