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Proceedings Paper

High Speed Striation Pattern Recognition In Contracting Cardiac Myocytes
Author(s): Kenneth P. Roos; A. Christyne Bliton; Bradford A. Lubell; John M. Parker; Mark J. Patton; Stuart R. Taylor
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Paper Abstract

The understanding of muscle contraction and relaxation requires the quantitation of movement at the sub-micron level in living cells. Two complementary non-RS-170 imaging systems used for authentic real time measurement of contractile dynamics are described and compared. Images from isolated skeletal or cardiac muscle cells are projected by an optical microscope onto single line or area charge-coupled device (CCD) photodiode arrays. These data are digitized and stored for subsequent image processing and analysis. The inherently low contrast muscle striation patterns are enhanced and their rapid movement measured with an accuracy at least an order of magnitude greater than traditional limits of optical resolution. The features of each image format are complementary and when combined provide the maximum overall information in time and space.

Paper Details

Date Published: 13 June 1989
PDF: 13 pages
Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); doi: 10.1117/12.951892
Show Author Affiliations
Kenneth P. Roos, The University of California (United States)
A. Christyne Bliton, The Mayo Foundation (United States)
Bradford A. Lubell, The University of California (United States)
John M. Parker, The University of California (United States)
Mark J. Patton, The Mayo Foundation (United States)
Stuart R. Taylor, The Mayo Foundation (United States)


Published in SPIE Proceedings Vol. 1063:
New Technologies in Cytometry
Gary C. Salzman, Editor(s)

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