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Proceedings Paper

Distances And Distance Distributions In A Ca[sup]2+[/sup]-Binding Protein
Author(s): Robert F. Steiner; Jianqing Lan; Louise Garone; Sharon Albaugh
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Paper Abstract

If a distribution of separations exists between a fluorescent donor group and an absorbing acceptor group, then the apparent efficiency of radiationless energy transfer will be different when monitored by measurements of static fluorescence intensity or average fluorescence decay time. This may be used to compute the parameters characterizing the distribution of separations, assuming it is Gaussian in form. Parameters computed in this way agree with those obtained by the frequency domain method for model systems. The distribution of separations has been computed for a donor group linked to Met-25 of troponin C and an acceptor group linked to Cys-98. The distribution narrows in the presence of Ca2+.

Paper Details

Date Published: 17 May 1989
PDF: 9 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951554
Show Author Affiliations
Robert F. Steiner, University of Maryland (United States)
Jianqing Lan, University of Maryland (United States)
Louise Garone, University of Maryland (United States)
Sharon Albaugh, University of Maryland (United States)


Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)

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