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Proceedings Paper

HPLC Determination Of Polycyclic Aromatic Compounds By Fluorescence Detected With A Charge-Coupled Device
Author(s): Rafi D. Jalkian; M. Bonner Denton
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Paper Abstract

A solid-state two-dimensional charge-coupled device (CCD) is used to quantitate polycyclic aromatic compounds by native fluorescence. The mixture is separated by reverse phase high performance liquid chromatography (HPLC) and the fluorescence emission from each peak is integrated by the detector. A new technique, charge-dependent variable binning, applied to one-dimensional spectroscopy leads to outstanding dynamic range. Excellent linearity and limits of detection are obtained for several priority pollutants. Synchronous and derivative techniques can be applied to the two-dimensional fluorescence data obtained to resolve overlapping peaks. The analyzed mixture consists of fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, and perylene.

Paper Details

Date Published: 17 May 1989
PDF: 12 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951546
Show Author Affiliations
Rafi D. Jalkian, University of Arizona (United States)
M. Bonner Denton, University of Arizona (United States)


Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)

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