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Proceedings Paper

Recovery Of Fluorescence Lifetime Distributions Generated By Heterogeneous Systems
Author(s): Aleksander Siemiarczuk; Brian D. Wagner; William R. Ware
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Paper Abstract

The Maximum Entropy Method and a new Exponential Series Method are described and tested for the recovery of underlying fluorescence lifetime distributions from digital fluorescence decay data. These two numerical techniques are applied to two heterogeneous systems, the intramolecular quenching in 1,3-di(1-pyrenyl)propane and the cupric ion quenching of pyrene in a sodium dodecyl sulfate micellar system. In the former, the short lifetime region is characterized by a broad distribution, which as the temperature is lowered, shifts dramatically to long lifetimes. In the micellar system, a lifetime distribution following the predicted Poisson distribution is recovered at each of five cupric ion concentrations, a result that supports the model of quencher ions distributed in the micelles according to Poisson statistics.

Paper Details

Date Published: 17 May 1989
PDF: 7 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951542
Show Author Affiliations
Aleksander Siemiarczuk, The University of Western Ontario (Canada)
Brian D. Wagner, The University of Western Ontario (Canada)
William R. Ware, The University of Western Ontario (Canada)


Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)

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