Share Email Print

Proceedings Paper

Using DNA looping to measure sequence dependent DNA elasticity
Author(s): Alan Kandinov; Krishnan Raghunathan; Jens-Christian Meiners
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We are using tethered particle motion (TPM) microscopy to observe protein-mediated DNA looping in the lactose repressor system in DNA constructs with varying AT / CG content. We use these data to determine the persistence length of the DNA as a function of its sequence content and compare the data to direct micromechanical measurements with constant-force axial optical tweezers. The data from the TPM experiments show a much smaller sequence effect on the persistence length than the optical tweezers experiments.

Paper Details

Date Published: 10 October 2012
PDF: 9 pages
Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 845816 (10 October 2012); doi: 10.1117/12.945930
Show Author Affiliations
Alan Kandinov, Univ. of Michigan (United States)
Krishnan Raghunathan, Univ. of Michigan (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8458:
Optical Trapping and Optical Micromanipulation IX
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

© SPIE. Terms of Use
Back to Top