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Proceedings Paper

Distributions In Phytoplankton Refractive Index And Size Within The North Sea
Author(s): Steven G Ackleson; David B Robins; John A Stephens
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Paper Abstract

A Coulter EPICS 741 Flow Cytometer was used at sea to measure distributions in phytoplankton refractive index and size within the North Sea. The optical data gathered by the flow cytometer, multi-band fluorescence and multi-angle light scatter, agreed well with coincident measurements of in vivo fluorescence. By gating on fluorescence, unique subpopulations of phytoplankton may be isolated optically from all other particles within a natural population. At several coastal stations, a unique subpopulation of phytoplankton was found which appeared to contain both chlorophyll and phycoerythrin. Using Mie scatter theory and flow cytometric measurements of light scatter in the near-forward and side directions, this population was found to be composed of cells of refractive index between 1.07 and 1.095 and spherical diameter between 7 and 9 pm (verified using Coulter volume measurements).

Paper Details

Date Published: 12 August 1988
PDF: 9 pages
Proc. SPIE 0925, Ocean Optics IX, (12 August 1988); doi: 10.1117/12.945739
Show Author Affiliations
Steven G Ackleson, Bigelow Laboratory for Ocean Science, (United States)
David B Robins, Natural Environmental Research Council (United States)
John A Stephens, Natural Environmental Research Council (United States)

Published in SPIE Proceedings Vol. 0925:
Ocean Optics IX
Marvin A. Blizard, Editor(s)

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