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Proceedings Paper

Error Analysis Of Phase Resolution Methods For Elimination Of Quenching Effects In Luminescence Spectroscopy
Author(s): J N Demas
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Paper Abstract

Earlier we described two methods of reducing quenching effects in analytical luminescence measurements based on phase resolved spectroscopy (PRS). These methods are a form of time resolution based on the amplitudes and the phase shifts of modulated signals. In Method 1 the signal is modulated at a high frequency, and the ac signal is essentially independent of the degree of sample quenching. Method 2 simultaneously measures the modulated amplitude and the phase shift between the emission and the excitation; from these data one can correct the signal to that of the unquenched sample. A procedure is given for calculating the precision and accuracy of both methods for different noise levels and degrees of sample quenching. The merits of both methods are discussed. Method 1 is shown to have a lower noise level than Method 2, but suffers from systematic errors especially at low modulation frequencies. Method 2 is superior if lower modulation frequencies must be used and computational complexity and speed of response are less critical.

Paper Details

Date Published: 27 April 1988
PDF: 6 pages
Proc. SPIE 0910, Fluorescence Detection II, (27 April 1988); doi: 10.1117/12.945456
Show Author Affiliations
J N Demas, University of Virginia (United States)


Published in SPIE Proceedings Vol. 0910:
Fluorescence Detection II
E. Roland Menzel, Editor(s)

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