Proceedings PaperError Analysis Of Phase Resolution Methods For Elimination Of Quenching Effects In Luminescence Spectroscopy
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Earlier we described two methods of reducing quenching effects in analytical luminescence measurements based on phase resolved spectroscopy (PRS). These methods are a form of time resolution based on the amplitudes and the phase shifts of modulated signals. In Method 1 the signal is modulated at a high frequency, and the ac signal is essentially independent of the degree of sample quenching. Method 2 simultaneously measures the modulated amplitude and the phase shift between the emission and the excitation; from these data one can correct the signal to that of the unquenched sample. A procedure is given for calculating the precision and accuracy of both methods for different noise levels and degrees of sample quenching. The merits of both methods are discussed. Method 1 is shown to have a lower noise level than Method 2, but suffers from systematic errors especially at low modulation frequencies. Method 2 is superior if lower modulation frequencies must be used and computational complexity and speed of response are less critical.