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Proceedings Paper

Effect Of Hydrostatic Pressure On Fluorescence Reactions In Proteins
Author(s): Maurice R Eftink; Zygmunt Wasylewski
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Paper Abstract

°The effect of hydrostatic pressure (0-2.6 kbar) on the acrylamide quenching of several single tryptophan containing proteins has been studied using phase fluorescence lifetime measurements, at 25C. For the model system, N-acetyl-L-tryptophanamide in water, we find essentially no dependence of the quenching rate constant, kq, on pressure. For the internal tryptophan residues of ribonuclease T1 and cod parvalbumin, we also find essentially no pressure dependence for the acrylamide kq. The low apparent activation volumes, LW*, characterize these quenching processes as involving very small amplitude fluctuations in the protein structures. Only for a poised monomer ↔tetramer equilibrium of melittin were we able to observe a significant effect of pressure on kq and this is due to the pressure induced shift in the equilibrium position.

Paper Details

Date Published: 24 June 1988
PDF: 5 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945418
Show Author Affiliations
Maurice R Eftink, University of Mississippi (United States)
Zygmunt Wasylewski, Jagellonian University (Poland)


Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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