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Proceedings Paper

Laser Spectroscopic Measurements Of Triplet-State Lifetimes In Both Time And Frequency Domains
Author(s): John J Birmingham; Peter B Garland
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Paper Abstract

We describe an apparatus for the measurement of slow rotational diffusion based on the phosphorescent photoluminescence of the triplet probe, erythrosin, and using phase modulation techniques. The apparatus has two novel features over those previously described: up to 40 frequencies of modulation are collected simultaneously, and the superimposition of fast 0-100% modulation of the excitation laser beam enables contaminating prompt fluorescence to be removed by time domain resolution during the frequency domain resolution of phosphorescence.

Paper Details

Date Published: 24 June 1988
PDF: 7 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945416
Show Author Affiliations
John J Birmingham, Dundee University (Scotland)
Peter B Garland, Unilever Research (England)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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