Share Email Print

Proceedings Paper

Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry
Author(s): lgnacy Gryczynski; Henryk Cherek; Joseph R Lakowicz
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We measured the frequency-response of the polarized emission of Yt-base in propylene glycol at 10°C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CC14, to decrease the mean decay time from 9.1 to 4.2 ns. These data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.

Paper Details

Date Published: 24 June 1988
PDF: 8 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945402
Show Author Affiliations
lgnacy Gryczynski, University of Maryland at Baltimore (United States)
Henryk Cherek, University of Maryland at Baltimore (United States)
Joseph R Lakowicz, University of Maryland at Baltimore (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

© SPIE. Terms of Use
Back to Top