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Proceedings Paper

Conformational Distributions Of Flexible Molecules From Steady State Fluorescence Energy Transfer
Author(s): Wieslaw Wiczk; Ignacy Grvczvnski; Michael L Johnson; Joseph R Lakowicz
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Paper Abstract

We demonstrate that distance distributions between two sites on flexible molecules can be recovered using steady state measurements of fluorescence energy transfer. The characteristic Forster distance (R°) for energy transfer was varied by collisional quenching of the donor, which decreases its quantum yield. The measured transfer efficiencies for each value of R. provide a different average of the distance distribution. The R.-dependent transfer efficiencies, when analyzed by non linear least- squares, were found to reliably determine the distance distribution, as shown by agreement with the frequency-domain method (Chem. Phys. Lett. 1987, 138:587-593).

Paper Details

Date Published: 24 June 1988
PDF: 7 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945401
Show Author Affiliations
Wieslaw Wiczk, University of Maryland at Baltimore (United States)
Ignacy Grvczvnski, University of Maryland at Baltimore (United States)
Michael L Johnson, University of Virginia School of Medicine (United States)
Joseph R Lakowicz, University of Maryland at Baltimore (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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