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Proceedings Paper

Submicrosecond Imaging Under A Pulsed-Laser Fluorescence Microscope
Author(s): Kazuhiko Kinosita; Ikuo Ashikawa; Masahiro Hibino; Masaya Shigemori; Hideyuki Yoshim ura; Hiroyasu Itoh; Kuniaki Nagayama; Akira lkegami
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Paper Abstract

A microscope system has been constructed that enables digital imaging of a fluorescent cell under pulsed illumination. Each image is produced by a single laser pulse of duration less than 0.3 11 s. With this system, microsecond responses of a single cell to an externally applied electric field have been resolved temporally and spatially. The cell membrane was stained with a voltage-sensitive fluorescent dye. The induction of transsmembrane potential by the applied field, and the perforation (electroporation) of the cell membrane under an intense field, were seen as successive images. The major finding was a transient increase, at the moment of perforation, in the membrane permeability to an enormous level in localized regions of the cell membrane. Possible roles in cell technology, as well as other applications of the microscope system, are discussed.

Paper Details

Date Published: 24 June 1988
PDF: 7 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945400
Show Author Affiliations
Kazuhiko Kinosita, The Institute of Physical and Chemical Research (Japan)
Ikuo Ashikawa, The Institute of Physical and Chemical Research (United States)
Masahiro Hibino, The Institute of Physical and Chemical Research (United States)
Masaya Shigemori, Tsukuba Research Laboratory (Japan)
Hideyuki Yoshim ura, JEOL Ltd. (Japan)
Hiroyasu Itoh, Tsukuba Research Laboratory (Japan)
Kuniaki Nagayama, JEOL Ltd. (Japan)
Akira lkegami, The Institute of Physical and Chemical Research (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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