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Proceedings Paper

Calcium Activation Profile In Electrically Stimulated Intact Rat Heart Cells
Author(s): Hugo Geerts; Rony Nuydens; Luc Ver Donck; Roger Nuyens; Marc De Brabander; Marcel Borgers
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Paper Abstract

Recent advances in fluorescent probe technology and image processing equipment have made available the measurement of calcium in living systems on a real-time basis. We present the use of the calcium indicator Fura-2 in intact normally stimulated rat heart cells for the spatial and dynamic measurement of the calcium excitation profile. After electric stimulation (1 Hz), the activation proceeds from the center of the myocyte toward the periphery. Within two frame times (80 ms), the whole cell is activated. The activation is slightly faster in the center of the cell than in the periphery. The mean recovery time is 200-400 ms. There is no difference along the cell's long axis. The effect of a beta-agonist and of a calcium antagonist is described.

Paper Details

Date Published: 24 June 1988
PDF: 5 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945396
Show Author Affiliations
Hugo Geerts, Janssen Research Foundation (Belgium)
Rony Nuydens, Janssen Research Foundation (Belgium)
Luc Ver Donck, Janssen Research Foundation (Belgium)
Roger Nuyens, Janssen Research Foundation (Belgium)
Marc De Brabander, Janssen Research Foundation (Belgium)
Marcel Borgers, Janssen Research Foundation (Belgium)


Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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