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Proceedings Paper

Emission Spectra Of Hemoglobin In The Picosecond Range
Author(s): Enrico Bucci; Henryk Malak; Clara Fronticelli; Ignacy Gryczynski; Joseph R Lakowicz
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Paper Abstract

We describe steady state emission spectra and fluorescent decay times from highly purified solutions of human hemoglobin, and the precautions necessary to obtain reliable measurements on the highly quenched intrinsic tryptophan emission. Hemoglobin displays a wide range of decay times (10 ps to 8 ns). The ps component are probably due to Hb itself, and the ns components are probably due to impurities. The ps emission appears to have emission maxima near 320 nm, which is characteristic of tryptophan residues which are shielded from contact with water. The ns components appear to have emission maxima near 340 nm. Our results indicate that the intrinsic emission of Hb can be a useful probe for its functional behavior.

Paper Details

Date Published: 24 June 1988
PDF: 8 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945387
Show Author Affiliations
Enrico Bucci, University of Maryland at Baltimore (United States)
Henryk Malak, University of Maryland at Baltimore (United States)
Clara Fronticelli, University of Maryland at Baltimore (United States)
Ignacy Gryczynski, University of Maryland at Baltimore (United States)
Joseph R Lakowicz, University of Maryland at Baltimore (United States)


Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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