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Proceedings Paper

Fluorescence Spectroscopy Data Analysis Environment: A Second Generation Global Analysis Program
Author(s): Joseph M Beechem; Enrico Gratton
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Paper Abstract

A fluorescence spectroscopy global analysis environment is described. Within this analysis environment, multidimensional fluorescence decay data (time and frequency domains) can be analyzed in terms of a wide variety of photophysical models. A generalized compartmental analysis structure is utilized, where one can specify the functions used to link the various compartments together. All fitting parameters may be characterized by either discrete or distributed values. Multi-temperature global analysis of frequency domain fluorescence studies from a single tryptophan containing protein are described.

Paper Details

Date Published: 24 June 1988
PDF: 12 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945370
Show Author Affiliations
Joseph M Beechem, The University of Illinois Urbana-Champaign (United States)
Enrico Gratton, The University of Illinois Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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