Share Email Print

Proceedings Paper

Technicon H*1 Hematology System: Optical Design Considerations
Author(s): G M Colella; D H Tycko; W Groner
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The Technicon H*1 systemTM is a clinical laboratory flow cytometer which performs a complete hematology profile, providing quantitative information on the various types of cells in a blood sample. A light-scattering method, using a HeNe laser, determines in a single flow channel the red cell count, platelet count, and the distributions of red cell volume, red cell hemoglobin concentration, and platelet volume. To accomplish this the scattered light from each red cell in the sample is measured in real time at two angular intervals. The cell volume and the hemoglobin concentration within the cell are derived from these two measurements. Severe accuracy and precision specifications are placed on the medically important red cell count (RBC) and the mean red cell volume (MCV). From the point of view of optical system design, the dominant factor is the requirement that RBC and MCV have precision and accuracy of the order of 2%. Signal-to-noise and scattering-angle definition requirements dictated the choice of a HeNe laser light source. The optics includes an illumination system for producing a sharply defined, uniformly illuminated scattering region and a detection system which must accurately define the accepted scattering angles. In previous cytometric methods for determining MCV only a single quantity was measured for each cell. Such methods cannot disentangle the independent effects of cell size and hemoglobin concentration on the measurement, thus compromising MCV accuracy. The present double-angle scattering method overcomes this accuracy problem. The H*1 red cell method, the supporting optical design and data demonstrating that the use of this technique eliminates interference between the observed red cell indices are presented.

Paper Details

Date Published: 15 June 1988
PDF: 9 pages
Proc. SPIE 0907, Laser Surgery: Characterization and Therapeutics, (15 June 1988); doi: 10.1117/12.945330
Show Author Affiliations
G M Colella, Technicon Instruments Corporation (United States)
D H Tycko, Technicon Instruments Corporation (United States)
W Groner, Technicon Instruments Corporation (United States)

Published in SPIE Proceedings Vol. 0907:
Laser Surgery: Characterization and Therapeutics
Kazuhiko Atsumi; Stephen N. Joffe, Editor(s)

© SPIE. Terms of Use
Back to Top