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Proceedings Paper

CCD Video Imaging Of Cardiac Activity
Author(s): G Nassif; F Fillette; Aouate G Lascault; Y Grosgogeat
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Paper Abstract

Helium-Neon LASER spectrometry of fluorescent dye WW 781 bound to heart tissues permits to collect optical signals significant from the electrical activity and from the electromechanical activity. It is also possible to image the electrical activity of a myocardial surface stained with WW 781 and illuminated with a direct or an unfocused Helium-Neon LASER beam using a Charge Coupled Device (CCD) video camera. Sheep ventricular fragments and right mouse atria were stained with Tyrode solutions containing from 0.4 to 1 g/1 WW 781. Focused or unfocused illuminations were performed with a 2 mW Helium-Neon LASER through a lens or an optical fiber. Direct illumination was performed with nine 5 mW Helim-Neon LASERs permitting to map the observed surface. CCD video camera was connected to a 70-220 Zoom-telelens and placed behind a 665 nm high pass optical filter. Video signals were amplified and recorded on a NTSC B. V. U professionnal magnetoscope. Video recordings were studied frame by frame. Fluorescent emissions from illuminated areas were monitored with a 200 um diameter optical fiber optrode using a monochromator-photomultiplier set. Direct illumination permitted to map on nine points and to follow the electrical activity propagation on sheep ventricular epicardium observing a 2/1 conduction block on a limited area. Fluorescent signals significant from electrical activity were simultaneously recorded. Unfocused lighting permitted to follow the depolarization of a 1.2 mm spot on sheep ventricular endocardium and to follow the propagation of the fluorescence on a 2 mm diameter area on mouse atrium. Such a technique appear to be of great imterest in the study of arrythmias on experimental models with foreseeable pharmacological applications.

Paper Details

Date Published: 15 June 1988
PDF: 7 pages
Proc. SPIE 0907, Laser Surgery: Characterization and Therapeutics, (15 June 1988); doi: 10.1117/12.945313
Show Author Affiliations
G Nassif, Hopital Pitie-Salpetriere (France)
F Fillette, Hopital Pitie-Salpetriere (France)
Aouate G Lascault, Hopital Pitie-Salpetriere (France)
Y Grosgogeat, Hopital Pitie-Salpetriere (France)

Published in SPIE Proceedings Vol. 0907:
Laser Surgery: Characterization and Therapeutics
Kazuhiko Atsumi; Stephen N. Joffe, Editor(s)

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