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Proceedings Paper

Enzyme-Based Fiber Optic Sensors
Author(s): Thomas J. Kulp; Irene Camins; Stanley M. Angel
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Paper Abstract

Fiber optic chemical sensors capable of detecting glucose and penicillin were developed. Each consists of a polymer membrane that is covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme and a pH-sensitive fluorescent dye (fluorescein). A signal is produced when the enzyme catalyzes the conversion of the analyte (glucose or penicillin) into a product (gluconic or penicilloic acid, respectively) that lowers the microenvironmental pH of the membrane and, consequently, lowers the fluorescence intensity of the dye. Each sensor is capable of responding to analyte concentrations in the range of ~0.1 to 100 mM. The penicillin optrode response time is 40 to 60 s while that for glucose is ~5 to 12 min.

Paper Details

Date Published: 21 June 1988
PDF: 5 pages
Proc. SPIE 0906, Optical Fibers in Medicine III, (21 June 1988); doi: 10.1117/12.945268
Show Author Affiliations
Thomas J. Kulp, Lawrence Livermore National Laboratory (United States)
Irene Camins, Lawrence Livermore National Laboratory (United States)
Stanley M. Angel, Lawrence Livermore National Laboratory (United States)


Published in SPIE Proceedings Vol. 0906:
Optical Fibers in Medicine III
Abraham Katzir, Editor(s)

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