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Proceedings Paper

Reconstruction Of 3D Light Microscopic Images
Author(s): Gerhard Zinser; Angelika Erhardt; Josef Bille
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Paper Abstract

Light-microscopic images of three-dimensional objects are distorted by defocused projections into the focus plane. A reconstruction procedure for three-dimensional light-microscopic images based on the linear system theory is described. Reconstruction is executed by inverse filtering of the image using an approximation of the three-dimen-sional optical transfer function of the microscope. The derivation and the features of this transfer function are out-lined. The reconstruction procedure permits to achieve a significant improvement of the image quality. The recon-struction result of a test object is shown.

Paper Details

Date Published: 4 December 1984
PDF: 6 pages
Proc. SPIE 0504, Applications of Digital Image Processing VII, (4 December 1984); doi: 10.1117/12.944882
Show Author Affiliations
Gerhard Zinser, German Cancer Research Center (West Germany)
Angelika Erhardt, German Cancer Research Center (West Germany)
Josef Bille, University of Heidelberg (West Germany)


Published in SPIE Proceedings Vol. 0504:
Applications of Digital Image Processing VII
Andrew G. Tescher, Editor(s)

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