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Proceedings Paper

Partial Inhibition Of Glycolysis With 2-Deoxy-D-Glucose Enhances EDKC Mediated Cellular Photosensitization.
Author(s): G. Ara; D. Ohuoha; A. R. Oseroff
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Paper Abstract

The effects of depleting glytolytic ATP on EDKC mediated cellular phototoxicity has been investigated using squamous carcinoma cells (FaDu) in vitro. Cells were pretreated with a non-metabolizable glucose analog 2-deoxy-D-glucose (5 mM) for 13-15 hr that resulted in 40-45 % decrease of cellular ATP. Subsequent exposure of the cells to EDKC resulted in twice as much dye uptake compared to control cells and upon radiation induced 4-5 fold more killing. Pretreatment with 2-deoxy-D-glucose (2-DG) alone did not have any effect on cell survival. We conclude that if cellular glycolytic ATP is lowered prior to their exposure to EDKC and radiation, cells become more vulnerable to the dye mediated photosensitization.

Paper Details

Date Published: 19 February 1988
PDF: 4 pages
Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); doi: 10.1117/12.942712
Show Author Affiliations
G. Ara, Tufts-New England Medical Center (United States)
D. Ohuoha, Tufts-New England Medical Center (United States)
A. R. Oseroff, Tufts-New England Medical Center (United States)

Published in SPIE Proceedings Vol. 0847:
New Directions in Photodynamic Therapy
Douglas C. Neckers, Editor(s)

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