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Proceedings Paper

Effect Of Free Radical Quenchers On Dye-Mediated Laser Light Induced Photosensitization Of Leukemic Cells
Author(s): Kirpal S. Gulliya; James Lester Matthews; Joseph Wayne Fay; Robert Morris Dowben
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Paper Abstract

The effect of free radical quenchers (ascorbate, catalase, and mannitol) on merocyanine 540 (MC540) mediated, laser light induced photolysis of human acute promyelocytic leukemia cell line (HL-60) was investigated. Results show that in the presence of human albumin (0.25%), dye-mediated (2014/m1), laser light induced photolysis of leukemic cells resulted in a 99.9999% cell kill. Seventy percent of the normal bone marrow cells survived the treatment. The addition of free radical quenchers prior to laser irradiation procedure increases the HL-60 cell survival. Increases of 5.5% and 4.4%, respectively, were observed in the presence of catalase and ascorbate or mannitol. In the presence of a mixture of catalase and mannitol or catalase and ascorbate, this increase in viability was not observed. However, the viability of normal bone marrow cells under these conditions also decreased from 70% to 63%. These findings may be useful in ex-vivo bone marrow purging.

Paper Details

Date Published: 19 February 1988
PDF: 3 pages
Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); doi: 10.1117/12.942706
Show Author Affiliations
Kirpal S. Gulliya, Baylor University Medical Center (United States)
James Lester Matthews, Baylor University Medical Center (United States)
Joseph Wayne Fay, Baylor University Medical Center (United States)
Robert Morris Dowben, Baylor University Medical Center (United States)

Published in SPIE Proceedings Vol. 0847:
New Directions in Photodynamic Therapy
Douglas C. Neckers, Editor(s)

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