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Proceedings Paper

Spectroscopy Of Nitrenes Bound To a-Chymotrypsin
Author(s): Matthew S. Platz; K. Kanakarajan; R. P. Goodrich; M. J. T. Young; S. Soundararajan
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Paper Abstract

Reaction of ∝-chymotrypsin with various aryl azido acylimidazoles leads to covalent attachment of the aryl azide photoaffinity label. Photolysis of the polycrystalline enzyme or a frozen solution of the enzyme at 77K leads to intense EPR signals of the triplet nitrenes. The position of the EPR resonance fields and the decay kinetics can be interpreted in terms of binding of the label within or outside the binding pocket of the enzyme.

Paper Details

Date Published: 19 February 1988
PDF: 4 pages
Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); doi: 10.1117/12.942690
Show Author Affiliations
Matthew S. Platz, The Ohio State University (United States)
K. Kanakarajan, The Ohio State University (United States)
R. P. Goodrich, The Ohio State University (United States)
M. J. T. Young, The Ohio State University (United States)
S. Soundararajan, The Ohio State University (United States)


Published in SPIE Proceedings Vol. 0847:
New Directions in Photodynamic Therapy
Douglas C. Neckers, Editor(s)

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