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Proceedings Paper

Photoaffinity Labeling As A Probe Of Ribosomal Structure And Function
Author(s): Barry S. Cooperman
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Paper Abstract

Results are presented showing how photoaffinity labeling can be used to identify ribosomal components at functional sites within the Escherichia coli ribosome. Following brief overviews of ribosomal structure and function and of the use of photoaffinity labeling to study complex biological macrostructures, results obtained in the authors' laboratory, principally with the ribosomal antibiotic puromycin and with an aryl azide derivative of puromycin, are presented to illustrate procedures used to identify photoaffinity-labeled ribosomal components, to determine whether such labeling occurs at a functionally important site, and to localize sites of labeling in a three-dimensional sense.

Paper Details

Date Published: 19 February 1988
PDF: 8 pages
Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); doi: 10.1117/12.942687
Show Author Affiliations
Barry S. Cooperman, University of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 0847:
New Directions in Photodynamic Therapy
Douglas C. Neckers, Editor(s)

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