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Proceedings Paper

A Real-Time Confocal Laser Scanning Microscope (CLSM)
Author(s): A. Draaijer; P. M. Houpt
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Paper Abstract

A serious disadvantage of most confocal optical scanning microscopes is that they use mechanical scanning methods, which lead to long frame times. Here we describe an off-axis CLSM in which scanning of the laser focal point is achieved with the aid of a fast acousto-optical deflector for line scanning, and a scanning mirror for frame scanning. This arrangement affords short frame-times of 0.05 seconds, and 0.02 seconds is within easy reach. A more or less three-dimensional image of the object can be built up at high speed by combining a number of confocal image sections. The system is suitable for reflection as well as fluorescence microscopy.

Paper Details

Date Published: 3 August 1987
PDF: 5 pages
Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); doi: 10.1117/12.941501
Show Author Affiliations
A. Draaijer, Netherlands Organization for Applied Scientific Research (Netherlands)
P. M. Houpt, Netherlands Organization for Applied Scientific Research (Netherlands)


Published in SPIE Proceedings Vol. 0809:
Scanning Imaging Technology
Ludwig J. Balk; Tony Wilson, Editor(s)

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