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Proceedings Paper

Scanning Optical Microscopy Applied To Fluorometry
Author(s): Gerard Roblin; Leon Bernstein
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Paper Abstract

Scanning Optical Microscopy, able to reconstruct, pixel after pixel, low noise images with the expected microscope resolution, is especially suitable for quantitative microscopy. Use of a bright, monochromatic spot of light extends its field of application to fluo-rescence Microscopy. Description of a typical device is given and the problems encountered to realize the scan of the laser beam are discussed. Results relating to transmitted light images as well as to epifluorescence images and spectral analysis are shown.

Paper Details

Date Published: 3 August 1987
PDF: 6 pages
Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); doi: 10.1117/12.941499
Show Author Affiliations
Gerard Roblin, Unite Associee au C.N.R.S. (France)
Leon Bernstein, Unite Associee au C.N.R.S. (France)


Published in SPIE Proceedings Vol. 0809:
Scanning Imaging Technology
Ludwig J. Balk; Tony Wilson, Editor(s)

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