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Proceedings Paper

Fiber Optic Immunodetectors: Sensors Or Dosimeters?
Author(s): J D Andrade; J-N Lin; J Herron; M Reichert; J Kopecek
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Paper Abstract

Biosensors based on specific interaction of the analyte of interest (ligand) with a biochemical agent (receptor) which specifically and tightly binds the analyte are being developed by many groups, agencies, and institutions using a variety of detection technologies. We are studying fluorescent methods using evanescent wave excitation on the surface of planar and cylindrical (fiber optic) optical waveguides. A relatively low ligand-receptor binding constant results in reversibility but low sensitivity. Most biological receptors bind very strongly and specifically--almost irreversibly--which provides an "irreversible" means of detection. Means to regulate ligand-receptor binding constants are discussed in order to provide semi-reversible, sensitive, specific sensors, rather than "one-shot" detectors or dosimeters.

Paper Details

Date Published: 28 April 1987
PDF: 6 pages
Proc. SPIE 0718, Fiber Optic and Laser Sensors IV, (28 April 1987); doi: 10.1117/12.937528
Show Author Affiliations
J D Andrade, University of Utah (United States)
J-N Lin, University of Utah (United States)
J Herron, University of Utah (United States)
M Reichert, University of Utah (United States)
J Kopecek, University of Utah (United States)

Published in SPIE Proceedings Vol. 0718:
Fiber Optic and Laser Sensors IV
Ramon P. DePaula; Eric Udd, Editor(s)

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