Share Email Print

Proceedings Paper

Fiber Optic Immunodetectors: Sensors Or Dosimeters?
Author(s): J D Andrade; J-N Lin; J Herron; M Reichert; J Kopecek
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Biosensors based on specific interaction of the analyte of interest (ligand) with a biochemical agent (receptor) which specifically and tightly binds the analyte are being developed by many groups, agencies, and institutions using a variety of detection technologies. We are studying fluorescent methods using evanescent wave excitation on the surface of planar and cylindrical (fiber optic) optical waveguides. A relatively low ligand-receptor binding constant results in reversibility but low sensitivity. Most biological receptors bind very strongly and specifically--almost irreversibly--which provides an "irreversible" means of detection. Means to regulate ligand-receptor binding constants are discussed in order to provide semi-reversible, sensitive, specific sensors, rather than "one-shot" detectors or dosimeters.

Paper Details

Date Published: 28 April 1987
PDF: 6 pages
Proc. SPIE 0718, Fiber Optic and Laser Sensors IV, (28 April 1987); doi: 10.1117/12.937528
Show Author Affiliations
J D Andrade, University of Utah (United States)
J-N Lin, University of Utah (United States)
J Herron, University of Utah (United States)
M Reichert, University of Utah (United States)
J Kopecek, University of Utah (United States)

Published in SPIE Proceedings Vol. 0718:
Fiber Optic and Laser Sensors IV
Ramon P. DePaula; Eric Udd, Editor(s)

© SPIE. Terms of Use
Back to Top