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Proceedings Paper

Fourier Transform Infrared (FTIR) Studies Of The Lipid Thermotropic Mesomorphism Of Acholeplasma laidlawii B
Author(s): Hector L. Casal; David G. Cameron; Adele Martin; Henry H. Mantsch
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Paper Abstract

The lipid components of the plasma membrane of the microorganism A. laidlawii B undergo a temperature induced phase transition. The biosynthetic incorporation of deuterated fatty acids into these membranes has allowed, via the C-D stretching vibrations, a detailed study of the lipid organization and dynamics, and the effect of the intrinsic membrane proteins on the transition. A number of fatty acids, of different chain lengths, have been incorporated biosynthetically in the presence or absence of avidin, a fatty acid synthesis inhibitor. In this manner the membranes are enriched solely in the added deuterated fatty acid up to high levels of homogeneity, providing an excellent system for the study of lipid-protein interactions. Detailed data regarding the lipid thermal behaviour will be presented and compared with results obtained by means of other techniques.

Paper Details

Date Published: 29 October 1981
PDF: 3 pages
Proc. SPIE 0289, 1981 Intl Conf on Fourier Transform Infrared Spectroscopy, (29 October 1981); doi: 10.1117/12.932159
Show Author Affiliations
Hector L. Casal, National Research Council of Canada (Canada)
David G. Cameron, National Research Council of Canada (Canada)
Adele Martin, National Research Council of Canada (Canada)
Henry H. Mantsch, National Research Council of Canada (Canada)

Published in SPIE Proceedings Vol. 0289:
1981 Intl Conf on Fourier Transform Infrared Spectroscopy
Hajime Sakai, Editor(s)

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