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Proceedings Paper

Biosensing with thermosensitive fluorescent quantum dot-containing polymer particles
Author(s): Alla N. Generalova; Vladimir A Oleinikov; Alyona Sukhanova; Mikhail V. Artemyev; Vitaly P. Zubov; Igor Nabiev
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Paper Abstract

In the past decades, increasing attention has been paid to the preparation of “smart” functionalized polymer particles reversibly responding to slight environmental changes, such as variations in temperature, pH, and ionic strength. The composite polymer particles consisting of a solid poly(acrolein-co-styrene) core and a poly(N-vinylcaprolactam) (PVCL) polymer shell doped with CdSe/ZnS semiconductor quantum dots (QDs) were prepared. The thermosensitive response of the composite particles was observed as a decrease in their hydrodynamic diameter upon heating above the lower critical solution temperature of the thermosensitive PVCL polymer used as a shell. Embedding QDs in the PVCL shell makes it possible to obtain particles whose fluorescence is sensitive to temperature changes. The temperature-dependent fluorescence of particles was determined by reversible variation of the distances between QDs in the PVCL shell as a result of temperature-driven conformational changes in this polymer. In addition, these particles can be used as carriers of biomolecule (e.g., bovine serum albumin, BSA) characterized by reversibly temperature-dependent fluorescence, which can serve as the basis for optical detection methods in bioassays, such as the measurement of local temperature in nanovolumes, biosensing, etc.

Paper Details

Date Published: 10 October 2012
PDF: 11 pages
Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600Y (10 October 2012); doi: 10.1117/12.931427
Show Author Affiliations
Alla N. Generalova, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Vladimir A Oleinikov, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Moscow Engineering Physics Institute (Russian Federation)
Alyona Sukhanova, Moscow Engineering Physics Institute (Russian Federation)
Trinity College Dublin (Ireland)
Mikhail V. Artemyev, Moscow Engineering Physics Institute (Russian Federation)
Belarusian State Univ. (Belarus)
Vitaly P. Zubov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Igor Nabiev, Moscow Engineering Physics Institute (Russian Federation)
Trinity College Dublin (Ireland)


Published in SPIE Proceedings Vol. 8460:
Biosensing and Nanomedicine V
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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