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Proceedings Paper

Photodynamic therapy with water-soluble phtalocyanines against bacterial biofilms in teeth root canals
Author(s): Raina Gergova; Tzvetelina Georgieva; Ivan Angelov; Vanya Mantareva; Serjoga Valkanov; Ivan Mitov; Slavcho Dimitrov
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Paper Abstract

The study presents the PDT with metal phthalocyanines on biofilms grown in root canals of ten representatives of the Gram-positive and the Gram-negative bacterial species and a fungus Candida albicans which cause aqute teeth infections in root canals.. The extracted human single-root teeth infected for 48 h with microorganisms in conditions to form biofilms of the above pathogens were PDT treated. The stage of biofilm formation and PDT effect of the samples of the teeth were determined by the scaning electron microscopy and with standard microbial tests. The PDT treating procedure included 10 min incubation with the respected phthalocyanine and irradiated with 660 nm Diode laser for 10 min. The most strongly antibacterial activity was achieved with zinc(II) phthalocyanine (ZnPc) against Enterococcus faecalis, Staphylococcus aureus and Moraxella catarrhalis. The other Gram-negative bacteria and Candida albicans were 10-100 times more resistant than the Gram-positive species. The Gram-negative Moraxella catarrhalis and Acinetobacter baumannii were more sensitive than the enterobacteria, but eradication of Pseudomonas aeruginosa in biofilm was insignificant. The influence of the stage of biofilm formation and the initial conditions (bacterial density, photosensitizer concentration and energy fluence of radiation) to the obtained level of inactivation of biofilms was investigated. The PDT with ZnPc photosensitizers show a powerful antimicrobial activity against the most frequent pathogens in endodontic infections and this method for inactivation of pathogens may be used with sucsses for treatment of the bacterial biofilms in the root canals.

Paper Details

Date Published: 8 May 2012
PDF: 12 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842744 (8 May 2012); doi: 10.1117/12.923859
Show Author Affiliations
Raina Gergova, Medical Univ. Sofia (Bulgaria)
Tzvetelina Georgieva, Medical Univ. Sofia (Bulgaria)
Ivan Angelov, Institute of Organic Chemistry with the Ctr. of Phytochemistry (Bulgaria)
Vanya Mantareva, Institute of Organic Chemistry with the Ctr. of Phytochemistry (Bulgaria)
Serjoga Valkanov, Institute of Metal Science (Bulgaria)
Ivan Mitov, Medical Univ. Sofia (Bulgaria)
Slavcho Dimitrov, Medical Univ. Sofia (Bulgaria)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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