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Proceedings Paper

Electronomicroscopic evaluation of the microlesional aspects in the pulp dentinal complex after repeated whitening therapy
Author(s): Rodica Bodea; Rodica Jianu; Cristian Marchese; Liliana Vasile
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Paper Abstract

The aim of this study was to examine cellular and matriceal dynamics within pulp tissue of the teeth with repeated bleaching. Material and method - The study was made on 25 patients aged between 15 and 45, to whom bleaching method of the premolars with indication of extraction in orthodontic purposes was applied. None of the subjects smoked and throughout the investigation no antibiotics had been used. We initiated an intensive oral hygiene program, and we removed the supragingival and subgingival deposits. Oral hygiene and the gingival health were evaluated before every session of bleaching. During each visit the dentition was cleaned professionally and if needed the subjects were reinstucted in proper oral hygiene. After 3 and 5 successive bleachings of the teeth, we removed the dental pulps and we extracted the premolars. The pulpal biopsies were fixed in buffed formaldehyde 10% for 48 hours, then paraffinized, sectioned at 3-5 μ and stained with topographic, H&E and trichrome stained. For the electonomicroscopic study we used the Lehner technique to process the biopsies (n=3) after the reinclusion of the pieces from the paraffine blocks in Epon, postfixated in buffered glutaraldehyde, micro sectioned at 0,5 μ, contrastated with Pb citrate (stained) and examination in transmission electronic microscopy with Philips microscope. Results - At cellular and matriceal level we observed a marked collagen fibrillogenesis in the presence of active fibroblasts, with well developed cellular organites and fibroclastic aspects which suggest matriceal active repair. The microvascular network presents an activated endothelium with turgescent endothelial cells, with intracitoplasmatic resorbtion vacuols, well developed Golgi Complex. Conclusion - We interpreed the cell - matriceal lesions in the context of the acute inflammatory process in the first lesional phase and chronic scleroatrophic process after successive bleaching.

Paper Details

Date Published: 8 May 2012
PDF: 8 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842741 (8 May 2012); doi: 10.1117/12.923661
Show Author Affiliations
Rodica Bodea, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania)
Rodica Jianu, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania)
Cristian Marchese, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania)
Liliana Vasile, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania)


Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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