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Proceedings Paper

Automated DNA-preparation system for bacteria out of air sampler liquids
Author(s): Rainer Gransee; Tina Röser; Klaus Stefan Drese; Dominik Düchs; Claudia Disqué; Gudrun Zoll; Stefan Köhne; Marion Ritzi-Lehnert
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Paper Abstract

Preventing bacterial contaminations is a significant challenge in applications across a variety of industries, e.g. in food processing, the life sciences or biohazard detection. Here we present a fully automated lab-on-a-chip system wherein a disposable microfluidic chip moulded by polymeric injection is inserted into an operating device. Liquid samples, here obtained from an air sampler, can be processed to extract and lyse bacteria, and subsequently to purify their DNA using a silica matrix. After the washing and elution steps, the DNA solution is dispensed into a reaction vessel for further analysis in a conventional laboratory polymerase chain reaction (PCR) device. We demonstrate the workability and efficiency of our approach with results from a 9 ml liquid sample spiked with E. coli.

Paper Details

Date Published: 16 May 2012
PDF: 9 pages
Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 83670G (16 May 2012); doi: 10.1117/12.923652
Show Author Affiliations
Rainer Gransee, Institut für Mikrotechnik Mainz GmbH (Germany)
Tina Röser, Institut für Mikrotechnik Mainz GmbH (Germany)
Klaus Stefan Drese, Institut für Mikrotechnik Mainz GmbH (Germany)
Dominik Düchs, Institut für Mikrotechnik Mainz GmbH (Germany)
Claudia Disqué, Molzym GmBH Co. KG (Germany)
Gudrun Zoll, Wehrwissenschaftliches Institut für Schutztechnologien (Germany)
Stefan Köhne, Wehrwissenschaftliches Institut für Schutztechnologien (Germany)
Marion Ritzi-Lehnert, Institut für Mikrotechnik Mainz GmbH (Germany)

Published in SPIE Proceedings Vol. 8367:
Smart Biomedical and Physiological Sensor Technology IX
Brian M. Cullum; Eric S. McLamore, Editor(s)

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