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Proceedings Paper

Video-based analysis of the rotational behaviour of rod-shaped, self-propelled bacteria in holographic optical tweezers
Author(s): Lena Dewenter; Christina Alpmann; Mike Woerdemann; Cornelia Denz
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Paper Abstract

Utilising the versatility of holographic optical tweezers and high speed video analysis, we present a scheme for the analysis of the rotational properties of multiple rod-shaped bacteria directly from video microscopy data. The bacterial body and flagella rotation frequency of Bacillus subtilis are determined by temporally resolved monitoring of the position of the bacterial body. In contrast to established methods, the video-based approach can be extended to the simultaneous analysis of several bacteria within the field of view. Monitoring multiple bacteria simultaneously will allow resolving the role of hydrodynamic interactions of multiple flagella motors on their mutual dynamics.

Paper Details

Date Published: 8 May 2012
PDF: 10 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84270N (8 May 2012); doi: 10.1117/12.922449
Show Author Affiliations
Lena Dewenter, Westfälische Wilhelms-Univ. Münster (Germany)
Christina Alpmann, Westfälische Wilhelms-Univ. Münster (Germany)
Mike Woerdemann, Westfälische Wilhelms-Univ. Münster (Germany)
Cornelia Denz, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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