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Proceedings Paper

Double optical fibre-probe device for the diagnosis of melanocytic lesions
Author(s): Riccardo Cicchi; Alessandro Cosci; Susanna Rossari; Vincenzo De Giorgi; Dimitrios Kapsokalyvas; Daniela Massi; Francesco S. Pavone
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Paper Abstract

We have designed and developed an optical fiber-probe for spectroscopic measurements on human tissues. The experimental setup combines fluorescence spectroscopy and Raman spectroscopy in a multidimensional approach. Concerning fluorescence spectroscopy, the excitation is provided by two laser diodes, one emitting in the UV (378 nm) and the other emitting in the visible (445 nm). These two lasers are used to selectively excite fluorescence from NADH and FAD, which are among the brightest endogenous fluorophores in human tissues. For Raman and NIR spectroscopy, the excitation is provided by a third laser diode with 785 nm excitation wavelength. Laser light is delivered to the tissue through the central optical fiber of a fiber bundle. The surrounding 48 fibers of the bundle are used for collecting fluorescence and Raman and for delivering light to the spectrograph. Fluorescence and Raman spectra are acquired on a cooled CCD camera. The instrument has been tested on fresh human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin, finding an optimal correlation with the subsequent histological exam. In some cases our examination was not in agreement with the clinical observation, but it was with the histological exam, demonstrating that the system can potentially contribute to improve clinical diagnostic capabilities and hence reduce the number of unnecessary biopsies.

Paper Details

Date Published: 8 May 2012
PDF: 8 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842714 (8 May 2012); doi: 10.1117/12.922405
Show Author Affiliations
Riccardo Cicchi, Univ. degli Studi di Firenze (Italy)
Alessandro Cosci, Univ. degli Studi di Firenze (Italy)
Susanna Rossari, Univ. degli Studi di Firenze (Italy)
Vincenzo De Giorgi, Univ. degli Studi di Firenze (Italy)
Dimitrios Kapsokalyvas, Univ. degli Studi di Firenze (Italy)
Daniela Massi, Univ. degli Studi di Firenze (Italy)
Francesco S. Pavone, Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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