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Proceedings Paper

Non-linear optical imaging and fibre-based spectroscopy of fresh colon biopsies
Author(s): R. Cicchi; A. Sturiale; G. Nesi; D. Kapsokalyvas; F. Tonelli; F. S. Pavone
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Paper Abstract

Two-photon fluorescence (TPEF) microscopy is a powerful tool to image human tissues up to 200 microns depth without any exogenously added probe. TPEF can take advantage of the autofluorescence of molecules intrinsically contained in a biological tissue, as such NADH, elastin, collagen, and flavins. Two-photon microscopy has been already successfully used to image several types of tissues, including skin, muscles, tendons, bladder. Nevertheless, its usefulness in imaging colon tissue has not been deeply investigated yet. In this work we have used combined two-photon excited fluorescence (TPEF), second harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two-photon emission detection (MTPE) to investigate different kinds of human ex-vivo fresh biopsies of colon. Morphological and spectroscopic analyses allowed to characterize both healthy mucosa, polyp, and colon samples in a good agreement with common routine histology. Even if further analysis, as well as a more significant statistics on a large number of samples would be helpful to discriminate between low, mild, and high grade cancer, our method is a promising tool to be used as diagnostic confirmation of histological results, as well as a diagnostic tool in a multiphoton endoscope or colonoscope to be used in in-vivo imaging applications.

Paper Details

Date Published: 8 May 2012
PDF: 7 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842736 (8 May 2012); doi: 10.1117/12.922388
Show Author Affiliations
R. Cicchi, Univ. degli Studi di Firenze (Italy)
A. Sturiale, Univ. degli Studi di Firenze (Italy)
G. Nesi, Univ. degli Studi di Firenze (Italy)
D. Kapsokalyvas, Univ. degli Studi di Firenze (Italy)
F. Tonelli, Univ. degli Studi di Firenze (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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