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Proceedings Paper

Use of Raman microspectroscopy to score the malignancy of breast cancer cells
Author(s): C. Nieva; M. Marro; R. Sanz-Pamplona; N. Santana-Codina; S. Rao; D. Petrov; A. Sierra
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Paper Abstract

It has been described that benign MCF10A breast cancer cell line suffers phenotypic changes toward malignancy when are cultured in sparse conditions. Using Raman spectroscopy with an InVia Raman microscope (Renishaw) with a backscattered configuration, we have studied the metabolic changes of confluent and sparse MCF10A cell cultures. We used Principal Component Analysis and Partial Least Squares Discriminant Analyses to assess the different profiling of the metabolic composition of breast cancer cells. The results indicated that Raman spectroscopy together with multivariate analysis is a useful technique to distinguish metabolic changes in malignant transformation. The identification of new metabolites, implementing the catalogue on the characterization of the different phenotypes associated to cell malignancy using Raman spectroscopy is under study.

Paper Details

Date Published: 8 May 2012
PDF: 6 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842713 (8 May 2012); doi: 10.1117/12.921356
Show Author Affiliations
C. Nieva, IDIBELL - Institut d'Investigació Biomèdica de Bellvitge (Spain)
ICFO - Institut de Ciències Fotòniques (Spain)
M. Marro, ICFO - Institut de Ciències Fotòniques (Spain)
R. Sanz-Pamplona, IDIBELL - Institut d'Investigació Biomèdica de Bellvitge (Spain)
N. Santana-Codina, IDIBELL - Institut d'Investigació Biomèdica de Bellvitge (Spain)
Univ. Autònoma de Barcelona (Spain)
S. Rao, ICFO - Institut de Ciències Fotòniques (Spain)
D. Petrov, ICFO - Institut de Ciències Fotòniques (Spain)
ICREA - Institució Catalana de Recerca i Estudis Avançats (Spain)
A. Sierra, IDIBELL - Institut d'Investigació Biomèdica de Bellvitge (Spain)


Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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