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Proceedings Paper

Novel utilization of the outer membrane proteins for the identification and differentiation of pathogenic versus nonpathogenic microbial strains using mass spectrometry-based proteomics approach
Author(s): Rabih E. Jabbour; Mary Wade; Samir V. Deshpande; Patrick McCubbin; A. Peter Snyder; Vicky Bevilacqua
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Paper Abstract

Mass spectrometry based proteomic approaches are showing promising capabilities in addressing various biological and biochemical issues. Outer membrane proteins (OMPs) are often associated with virulence in gram-negative pathogens and could prove to be excellent model biomarkers for strain level differentiation among bacteria. Whole cells and OMP extracts were isolated from pathogenic and non-pathogenic strains of Francisella tularensis, Burkholderia thailandensis, and Burkholderia mallei. OMP extracts were compared for their ability to differentiate and delineate the correct database organism to an experimental sample and for the degree of dissimilarity to the nearest-neighbor database strains. This study addresses the comparative experimental proteome analyses of OMPs vs. whole cell lysates on the strain-level discrimination among gram negative pathogenic and non-pathogenic strains.

Paper Details

Date Published: 5 May 2012
PDF: 10 pages
Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 835809 (5 May 2012); doi: 10.1117/12.921254
Show Author Affiliations
Rabih E. Jabbour, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Mary Wade, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Samir V. Deshpande, Science & Technology Corp. (United States)
Patrick McCubbin, OptiMetrics, Inc. (United States)
A. Peter Snyder, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Vicky Bevilacqua, U.S. Army Edgewood Chemical Biological Ctr. (United States)


Published in SPIE Proceedings Vol. 8358:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Augustus Way Fountain, Editor(s)

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