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Proceedings Paper

New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling
Author(s): Shyamala Pillai; Laura Wirpsza; Maxim Kozlov; Salvatore A. E Marras; Lev N. Krasnoperov; Arkady Mustaev
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Paper Abstract

New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.

Paper Details

Date Published: 2 February 2012
PDF: 12 pages
Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331C (2 February 2012); doi: 10.1117/12.921056
Show Author Affiliations
Shyamala Pillai, New Jersey Institute of Technology (United States)
New Jersey Medical School (United States)
Laura Wirpsza, New Jersey Institute of Technology (United States)
New Jersey Medical School (United States)
Maxim Kozlov, New Jersey Medical School (United States)
Enghelhardt Institute of Molecular Biology (Russian Federation)
Salvatore A. E Marras, New Jersey Medical School (United States)
Lev N. Krasnoperov, New Jersey Institute of Technology (United States)
Arkady Mustaev, New Jersey Medical School (United States)


Published in SPIE Proceedings Vol. 8233:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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