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Proceedings Paper

Impedance biosensor based on interdigitated electrode array for detection of E.coli O157:H7 in food products
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Paper Abstract

An impedance biosensor was designed, fabricated and tested for detection of viable Escherichia coli O157:H7 in food samples. This device consists of interdigitated microelectrode array (IDEA) fabricated using thin layer of sputtered gold, embedded under a polydimethylsiloxane (PDMS) microchannel. The array of electrodes is designed to detect viable EColi in different food products. The active surface area of the detection array was modified using goat anti-E.coli polyclonal IgG antibody. Contaminated food samples were tested by infusing the supernatant containing bacteria over the IDEA's, through the microchannel. Antibody-antigen binding on the electrodes results in impedance change. Four serial concentrations of E.coli contaminated food samples (3x102 CFUmL-1 to 3x105 CFUmL-1) were tested. The biosensor successfully detected the E.coli samples, with the lower detection limit being 3x103 CFUmL-1 (up to 3cells/μl). Comparing the test results with an IDEA impedance biosensor without microchannel (published elsewhere) indicates that this biosensor have two order of magnitude times higher sensitivity. The proposed biosensor provides qualitative and quantitative detection, and potentially could be used for detection of other type of bacteria by immobilizing the specific type of antibody.

Paper Details

Date Published: 4 May 2012
PDF: 7 pages
Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690Q (4 May 2012); doi: 10.1117/12.920821
Show Author Affiliations
Shibajyoti Ghosh Dastider, Univ.of Missouri-Columbia (United States)
Syed Barizuddin, Lincoln Univ. (United States)
Majed Dweik, Lincoln Univ. (United States)
Mahmoud F. Almasri, Univ. of Missouri-Columbia (United States)

Published in SPIE Proceedings Vol. 8369:
Sensing for Agriculture and Food Quality and Safety IV
Moon S. Kim; Shu-I Tu; Kuanglin Chao, Editor(s)

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