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Proceedings Paper

Development of bacterial display peptides for use in biosensing applications
Author(s): Dimitra N. Stratis-Cullum; Joshua M. Kogot; Michael S. Sellers; Margaret M. Hurley; Deborah A. Sarkes; Joseph M. Pennington; Irene Val-Addo; Bryn L. Adams; Candice R. Warner; James P. Carney; Rebecca L. Brown; Paul M. Pellegrino
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Paper Abstract

Recent advances in synthetic library engineering continue to show promise for the rapid production of reagent technology in response to biological threats. A synthetic library of peptide mutants built off a bacterial host offers a convenient means to link the peptide sequence, (i.e., identity of individual library members) with the desired molecular recognition traits, but also allows for a relatively simple protocol, amenable to automation. An improved understanding of the mechanisms of recognition and control of synthetic reagent isolation and evolution remain critical to success. In this paper, we describe our approach to development of peptide affinity reagents based on peptide bacterial display technology with improved control of binding interactions for stringent evolution of reagent candidates, and tailored performance capabilities. There are four key elements to the peptide affinity reagent program including: (1) the diverse bacterial library technology, (2) advanced reagent screening amenable to laboratory automation and control, (3) iterative characterization and feedback on both affinity and specificity of the molecular interactions, and (3) integrated multiscale computational prescreening of candidate peptide ligands including in silico prediction of improved binding performance. Specific results on peptides binders to Protective Antigen (PA) protein of Bacillus anthracis and Staphylococcal Enterotoxin B (SEB) will be presented. Recent highlights of on cell vs. off-cell affinity behavior and correlation of the results with advanced docking simulations on the protein-peptide system(s) are included. The potential of this technology and approach to enable rapid development of a new affinity reagent with unprecedented speed (less than one week) would allow for rapid response to new and constantly emerging threats.

Paper Details

Date Published: 5 May 2012
PDF: 9 pages
Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 83580A (5 May 2012); doi: 10.1117/12.919782
Show Author Affiliations
Dimitra N. Stratis-Cullum, U.S. Army Research Lab. (United States)
Joshua M. Kogot, U.S. Army Research Lab. (United States)
Michael S. Sellers, U.S. Army Research Lab. (United States)
Margaret M. Hurley, U.S. Army Research Lab. (United States)
Deborah A. Sarkes, U.S. Army Research Lab. (United States)
Joseph M. Pennington, U.S. Army Research Lab. (United States)
Irene Val-Addo, U.S. Army Research Lab. (United States)
Bryn L. Adams, U.S. Army Research Lab. (United States)
Candice R. Warner, U.S. Army Edgewood Chemical Biological Ctr. (United States)
James P. Carney, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Rebecca L. Brown, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Paul M. Pellegrino, U.S. Army Research Lab. (United States)


Published in SPIE Proceedings Vol. 8358:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Augustus Way Fountain, Editor(s)

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