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Proceedings Paper

Classification of Shiga toxin-producing escherichia coli (STEC) serotypes with hyperspectral microscope imagery
Author(s): Bosoon Park; William R. Windham; Scott R. Ladely; Prudhvi Gurram; Heesung Kwon; Seung-Chul Yoon; Kurt C. Lawrence; Neelam Narang; William C. Cray
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Paper Abstract

Non-O157:H7 Shiga toxin-producing Escherichia coli (STEC) strains such as O26, O45, O103, O111, O121 and O145 are recognized as serious outbreak to cause human illness due to their toxicity. A conventional microbiological method for cell counting is laborious and needs long time for the results. Since optical detection method is promising for realtime, in-situ foodborne pathogen detection, acousto-optical tunable filters (AOTF)-based hyperspectral microscopic imaging (HMI) method has been developed for identifying pathogenic bacteria because of its capability to differentiate both spatial and spectral characteristics of each bacterial cell from microcolony samples. Using the AOTF-based HMI method, 89 contiguous spectral images could be acquired within approximately 30 seconds with 250 ms exposure time. From this study, we have successfully developed the protocol for live-cell immobilization on glass slides to acquire quality spectral images from STEC bacterial cells using the modified dry method. Among the contiguous spectral imagery between 450 and 800 nm, the intensity of spectral images at 458, 498, 522, 546, 570, 586, 670 and 690 nm were distinctive for STEC bacteria. With two different classification algorithms, Support Vector Machine (SVM) and Sparse Kernel-based Ensemble Learning (SKEL), a STEC serotype O45 could be classified with 92% detection accuracy.

Paper Details

Date Published: 5 May 2012
PDF: 13 pages
Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690L (5 May 2012); doi: 10.1117/12.919695
Show Author Affiliations
Bosoon Park, USDA Agricultural Research Service (United States)
William R. Windham, USDA Agricultural Research Service (United States)
Scott R. Ladely, USDA Food Safety and Inspection Service (United States)
Prudhvi Gurram, U. S. Army Research Lab. (United States)
Heesung Kwon, U. S. Army Research Lab. (United States)
Seung-Chul Yoon, USDA Agricultural Research Service (United States)
Kurt C. Lawrence, USDA Agricultural Research Service (United States)
Neelam Narang, USDA Food Safety and Inspection Service (United States)
William C. Cray, USDA Food Safety and Inspection Service (United States)


Published in SPIE Proceedings Vol. 8369:
Sensing for Agriculture and Food Quality and Safety IV
Moon S. Kim; Shu-I Tu; Kuanglin Chao, Editor(s)

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