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Proceedings Paper

Direct imaging of macrophage activation during PDT treatment
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Paper Abstract

Mounting evidence describes a more complex progress of macrophage activation during photodynamic therapy (PDT), which performing distinct immunological functions and different physiologies on surrounding cells and tissues. Macrophage-targeted PDT has been applied in the selective killing of cells involved in inflammation and tumor. We have previously shown that PDT-mediated tumor cells apoptosis can induce a higher level immune response than necrosis, and enhance the macrophage activation. However, the molecular mechanism of macrophage activation during PDT-induced apoptotic cells (AC) still unclear. Here, we use confocal microscopy to image the phagocytosis of tumor cells by macrophages. We also observed that PDT-treated AC can activate Toll-like receptors (TLRs) which are present on macrophages surface. Besides, the increase in nitric oxide (NO) formation in macrophages was detected in real time by a laser scanning microscopy. This study provided more details for understanding the molecular mechanism of the immune response induced by PDT-treated AC.

Paper Details

Date Published: 13 March 2012
PDF: 10 pages
Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290M (13 March 2012); doi: 10.1117/12.919005
Show Author Affiliations
Sheng Song, South China Normal Univ. (China)
Feifan Zhou, South China Normal Univ. (China)
Wei R. Chen, South China Normal Univ. (China)
Univ. of Central Oklahoma (United States)
Da Xing, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 8329:
Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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