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Proceedings Paper

Immunoassay for CEA using the novel probe-labeled Ag nanoparticles based on surface-enhanced Raman scattering
Author(s): Xiaoqian Lin; Gangqin Xi; Yanping Chen; Gang Chen; Xiongwei Zheng; Jinping Lei; Lin Ou; Shangyuan Feng; Haishan Zeng; Rong Chen; Liqing Sun
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Paper Abstract

In this study, we report the Ag nanoparticles aggregated in the process of the labeling that use the crystal violet as the Raman probe. After this process- immune nanoparticles aggregate with high SERS sensitivity and biological specificity are created. We track and characterize the preparation by employing UV-Vi absorption spectra, Transmission Electron Microscope (TEM) and SERS spectra. With the increase of crystal violet, the aggregate of the Ag nanoparticles also increase, while the intensity of absorption peaks decrease. When the concentration of crystal violet reaches 1.0×10-4 mol/L, new peaks were found in the long wavelengths, and with the increase of the crystal violet, the intensity of the new peaks increase as well. We observe from the TEM, that with the increase of crystal violet, the aggregation degree of the Ag nanoparticles also increase and then they unite together. The SERS activity of the aggregates was directly related to the aggregation degree. Detecting the SERS activity of Ag NPs aggregates labeled with different amount of crystal violet, we found that with the increase of the crystal violet, the SERS signals of NPs aggregates enhanced. However, when the amount of crystal violet exceeded 25μL in 1mL colloidal silver, the Ag NPs occurred agglomeration and thereafter the next preparation of immune-label aggregates was hindered. Whereas, the probe labelled with 12μL crystal violet exhibited a better stability, stronger SERS activity and higher biological specificity, and it may accomplish a highly efficient SERS-based immunoassay. This immune probe was applied for detecting the expression of carcinoembryonic antigen (CEA) in the colon cancer tissue slice. Results show that appropriate immune aggregates labelled with optimum quantity of crystal violet present high stability, strong SERS activity and good immune specificity, which are expected to be applied in the analysis of the protein expression in the tissue section and promising for developing into a clinical tool for diagnosis.

Paper Details

Date Published: 13 March 2012
PDF: 6 pages
Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290J (13 March 2012); doi: 10.1117/12.918954
Show Author Affiliations
Xiaoqian Lin, Fujian Normal Univ. (China)
Gangqin Xi, Fujian Normal Univ. (China)
Yanping Chen, Fujian Provincial Tumor Hospital (China)
Gang Chen, Fujian Provincial Tumor Hospital (China)
Xiongwei Zheng, Fujian Provincial Tumor Hospital (China)
Jinping Lei, Fujian Normal Univ. (China)
Lin Ou, Fujian Normal Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Haishan Zeng, British Columbia Cancer Agency Research Ctr. (Canada)
Rong Chen, Fujian Normal Univ. (China)
Liqing Sun, Fuzhou First Hospital (China)

Published in SPIE Proceedings Vol. 8329:
Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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