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Proceedings Paper

Development of a simple and rapid assay for methylase activity based on DNA hairpin probe and Sybr Green I
Author(s): Yi Long; Xiaoming Zhou
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Paper Abstract

Methylase is vital for a large number of biological reactions. Here we developed a new method for DNA methylase activity analysis. In this paper, a DNA hairpin probe with a sequence of 5'-GATC-3' in the stem region was designed. The 5'-GATC-3' sequence was targeted by Dam MTase and was methylated. Subsequently, restriction enzyme Dpnl recognized the site and cut it. Then the haipin probe was transformed into three single stranded DNA. This enzymatic process can be monitored by the change of SYBR green I fluorescence. The current label free assay is an useful tool for DNA methylase activity analysis due to its simplicity, speedability, and low cost.

Paper Details

Date Published: 14 March 2012
PDF: 5 pages
Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290C (14 March 2012); doi: 10.1117/12.918829
Show Author Affiliations
Yi Long, South China Normal Univ. (China)
Xiaoming Zhou, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 8329:
Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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